摘要
目的:建立以高效液相色谱(HPLC)法同时测定几种抗骨质疏松类中成药中柚皮苷及淫羊藿苷含量的方法。方法:色谱柱为InertsilC1(8250mm×4.6mm,5μm),流动相为乙腈-水(梯度洗脱),流速为1mL·min-1,检测波长为285nm。结果:柚皮苷进样量在0.0874~8.74μg范围内与峰面积积分值呈良好线性关系(r=0.9999),加样回收率在99.3%~103.8%之间,RSD为2.18%~3.01%(n=6);淫羊藿苷进样量在0.0606~6.06μg范围内与峰面积积分值呈良好线性关系(r=0.9999),加样回收率在95.9%~98.6%之间,RSD为0.97%~1.66%(n=6)。结论:本法简便、准确、稳定、重复性好,可为评价含淫羊藿、骨碎补中药制剂的质量提供参考。
OBJECTIVE:To establish a HPLC method for the determination of icariin and naringin in anti-osteoporosis Chinese formulated products.METHODS:Inertsil C 18(250 mm×4.6 mm,5μm)column was used with a mobile phase of acetonitrile-water(gradient elution)at the flow rate of 1 mL·min -1.The detection wavelength was set at 285 nm.RESULTS:The linear rang of naringin was 0.0606~6.06μg(r=0.999 9)with average recovery of 95.9%~98.6%(RSD ranged from 0.97% to 1.66%,n=6).The linear rang of icariin was 0.087 4~8.74μg(r=0.999 9)with average recovery of 99.3%~103.8%(RSD ranged from 2.18% to 3.01%,n=6).CONCLUSION:The method is simple,reliable,accurate and reproducible for quality control of Chinese formulated products.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第43期4083-4085,共3页
China Pharmacy
基金
"十一五"军队中医药研发推广专项课题(2006062005)