摘要
目的:探讨EGF和TGF-α影响舌苔形成的分子机制。方法:MTT法、流式细胞术、细胞免疫组化研究EGF和TGF-α对原代大鼠舌背黏膜上皮细胞增殖、周期与凋亡、MAPK信号通路分子表达的影响。结果:EGF和TGF-α对细胞的作用效果类似,但强度不同,差异在于TGF-α刺激细胞有丝分裂的能力更强。结论:EGF和TGF-α是影响舌苔形成的关键分子。
Objective:To explore the molecular mechanism of EGF and TGF-??regulating the formation of tongue coatings. Methods:Lingual dorsal epithelial cells were isolated cultured in vitro,MTT method,flow cytometric analysis and immunohisto-chemistry were employed to investigate the cell proliferation,cell cycle distribution and apoptosis,and expressed levels of several key molecules in MAPK signal pathway. Results:EGF and TGF-??showed similar effects on the lingual dorsal epithelial cells,but the intensity was different. Conclusion:EGF and TGF -??may be the two key molecules in the formation of tongue coating.
出处
《辽宁中医杂志》
CAS
北大核心
2010年第11期2091-2094,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(30472123,30973715)
江苏省自然科学基金(BK2008461)
南京中医药大学青年自然科学基金(07XZR01)