急性胰腺炎时骨髓和循环内皮祖细胞的变化研究

Changes of bonemarrow and circulating endothelial progenitor cells in mice with acute pancreatitis

目的探讨急性胰腺炎时骨髓及循环内皮祖细胞（EPCs）的数量变化及机制。方法将BALB／c小鼠随机分为对照组和腹腔注射雨蛙素组，腹腔注射后12h、24h、48h，用流式细胞仪测定骨髓和循环中EPCs数量，分别采用ELISA法和Western lot检测循环中VEGF、TNF-α、ET-1含量及胰腺VEGF表达，TUNEL法原位检测胰腺血管内皮细胞凋亡。结果腹腔内雨蛙素注射后骨髓和循环中EPCs的数量显著增加（P〈0．05）并伴有血浆高水平的VEGF、TNF—α、ET，1（P〈0．05），骨髓和循环中EPCs数量与血浆VEGF水平呈线性相关（r分别为0．77和0．67）；雨蛙素注射后12h胰腺VEGF表达明显增加；雨蛙素注射后24h胰腺血管内皮细胞凋亡明显增加。结论急性胰腺炎可导致胰腺局部血管内皮损伤，并可动员骨髓内EPCs进入循环。

Objective To investigate changes in number of endothelial progenitor cells （ EPCs ） from bone marrow and circulation in mice with acute pancreatitis. Methods BALB/c mice were assigned randomly to saline group and cerulein group. Animals were sacrificed at 12, 24 and 48 hours after injection. Bone marrow and circulating EPCs were detected by flow cytometric analysis. Plasma VEGF, TNF-α and ET-1 were determined by enzyme-linked immunosorbent assay. The expression of VEGF in the pancreas was assessed by Western blotting. Apoptosis in situ was detected by TUNEL. Results The amounts of EPCs in bone marrow and circulation increased remarkably after cerulein injection （ P 〈 0.05 ）, also the levels of plasma VEGF TNF-α and ET-1 （ P 〈 0.05 ）, the EPCs levels in bone marrow and circulation seen in the study closely mirrors the levels of VEGF detected in the circulation （ r = 0.77, 0.67 individually）. VEGF expression in pancreas was up-regulated after 12 h of cerulein injection compared with that of control group. Apoptosis of endothelial ceils also increased in the cerulein group. Conclusion EPCs were mobilized by acute pancreatitis, which may be due to the mobilizing effect of increased levels of VEGF, EPCs may participate in the repair process of injured endothelium induced by acute pancreatitis.