核心蛋白聚糖在移植肝慢性排斥反应中的表达及意义

The expression and significance of decorin in chronic rejection of liver transplantion

目的探讨核心蛋白聚糖（DCN）在移植肝慢性排斥反应（CR）患者肝组织及血清中的表达及意义。方法以免疫组织化学（sP法）检测正常人16例、肝硬化20例、移植肝无CR46例及合并CR8例患者肝组织中DCN的表达情况，以酶联免疫方法（Elisa法）测定各组血清中DCN的含量情况。结果移植肝并CR与无CR组织及正常组织中DCN均呈阴性表达，肝硬化组织中呈阳性表达，移植肝并CR阳性表达率、平均吸光度与肝硬化组织相比，有显著性差异（25％US55％，0．1249±0．0039US0．2357±0．0396，P〈0．01），与正常肝及移植肝无CR组相比，差异无统计学意义；移植肝并CR患者血清中DCN含量明显升高，与正常人、肝硬化及移植肝无CR组相比，有显著性差异（54．0833±6，0325μg／L vs 1．0232±0．9105μg／L，12．6202±1．5370μg／L，17．7102±2．3562μg／L，P〈0．01），DCN血清浓度与排斥反应程度呈正相关。结论DCN在移植肝并CR患者肝组织中呈阴性表达，而在血清中呈明显升高，提示其可能参与CR的发生、发展，同时血清DCN测定有可能成为移植肝CR早期诊断、发展及预后的非创伤性重要指标。

Objective To investigate expression and significance of decorin （DCN） in liver tissue and serum of liver transplant patients with chronic rejection （CR）. Methods Immunohistochemistry （ SP method） was used to detect expression of DCN in liver tissue of 16 normal controls, 20 patients with cirrhosis, 46 liver translantion patients without CR and 8 patients with CR. Enzyme-linked immunosorbent assay method （ELISA method） was used to determined the content of DCN in serum of all research subjects. Results The expression of DCN was negative in normal hepatic tissues and with/without CR, cirrhosis tissues showed strong expression of DCN. The positive expression rate and the average optical density value of DCN in liver transplant tissues with CR had significant difference comparing with Cirrhosis tissues （25% vs 55% , 0. 1249 ±0.0039 vs 0. 2357 ±0. 0396, P 〈0.01 ,while no statistic siqnificance compared to normal liver tissues and those without CR. The level of DCN in serum was significantly higher in liver transplant patients with CR, with significant differenee comparing with normal people, liver cirrhosis and transplant liver patients without CR（54.0833 ± 6. 0325 ） μg/L vs （ 1. 0232 ± 0. 9105 ） μg/L, （ 12. 6202 ± 1. 5370） μg/L, （ 17. 7102 ±2.3562） μg/L, P 〈 0.01 ）. The concentration of DCN in serum showed a positive correlation with the degree of CR. Conclusions DCN showed negative expression in liver tissue and increased significantly in serum of liver transplantation patients with CR. This suggests that DCN may be involved in occurrence and development of CR. At the same time the determination of DCN in serum maybe become an important indicator of the early diagnosis, development and prognosis of CR for liver transplant patients.