微生物源性抗氧化剂对大鼠抗氧化及损伤修复的作用

Antioxidation and damage repair ability of microbe-derived antioxidants in rats

本试验以脂多糖(LPS)致氧化损伤大鼠为模型,研究微生物源性抗氧化剂对大鼠生长性能、脏器系数、抗氧化及损伤修复的作用。将64只70日龄SD大鼠随机分成4组,每组16只,雌雄对半。对照组和损伤模型组饲喂基础日粮,抗氧化剂组和损伤修复组饲喂基础日粮并添加0.8 mL/d.只微生物源性抗氧化剂。损伤模型组和损伤修复组腹腔注射LPS 1 mg/kg,对照组和抗氧化剂组注射等量的生理盐水,试验期22 d。结果显示,与对照组相比,抗氧化剂显著增加大鼠日增重,提高血清超氧化物岐化酶(SOD)活力,降低丙二醛(MDA)含量和一氧化氮(NO)水平(P<0.05),对脏器系数影响不显著。与损伤模型组相比,损伤修复组可提高大鼠抗氧化性能,修复损伤使大鼠抗氧化性能恢复至对照组水平,表明微生物源性抗氧化剂具有提高大鼠抗氧化性能、修复机体氧化损伤的作用。

The objective of the research was to determine the effects of microbe-derived antioxidants on antioxidation and damage repair ability in oxidative damage model rats induced by lipopolysaccharide（LPS）.Sixty four 70-day SD rats were randomly divided into four groups with 16 replicates of each treatment.Control group and model group were fed basal diet,antioxidant group and repair group were fed basal diet supplemented with 0.8 mL/d microbe-derived antioxidants.Model and repair group were injected 1 mg/kg LPS through intraperitoneal injection,and control and antioxidant group were injected normal saline.The trial period was 22 days.The results showed that compared with control group,antioxidant supplementation could significantly increase average daily gain and SOD activity,decrease MDA concentration,and reduce NO level（P〈 0.05）.There was no significant difference in the relative weight of inner organs（P 〉0.05）.Compared with model group,repair group supplemented with microbe-derived antioxidants could improve antioxidation capacity and repair oxidative damage of model rats.It could be concluded that microbe-derived antioxidants had the effects of antioxidation to positively modulate free radicals metabolism and repair oxidative damage of rats.