同步荧光扫描导数法测定延胡索药材中原阿片碱含量

Determination of Opioid Alkaloid in Rhizoma Corydalis by Synchronous Fluorescence Derivative Method

目的:建立同步荧光扫描导数法测定延胡索药材中原阿片碱含量的方法。方法:采用同步荧光扫描导数法以Δλ=10 nm,激发波长扫描范围200～400 nm,扫描结果经一阶导数处理,在290 nm峰位波长处采用标准加入法测定原阿片碱的荧光强度。结果:原阿片碱在1.6～12.00μg.mL-1浓度范围之间线性关系良好,r=0.9991。原阿片碱加样回收率为95.2%,RSD为1.1%。结论:该方法快速准确,灵敏度高,选择性好,可用于延胡索药材中原阿片碱的含量测定。

Objective：To develop a synchronous fluorescence derivative method for the determination of opioid alkaloid in rhizoma corydalis.Methods：Synchronous fluorescence scan derivative method was applied for the measurement of the fluorescence strength of opioid alkaloid with Δλ = 10 nm,Ex scanning range was from 200 nm to 400 nm.The scan spec-trum was conducted by one step derivative,and the fluorescence intensity was detected at 290 nm.The quantitation of opi-oid alkaloid was conducted with standard addition method.Results：Opioid alkaloid has shown good linear relationship be-tween fluorescence intensity and concentration in the range of 1.6 ～ 12.0 μg.mL-1,r = 0.9991.The average rate of re-covery was 95.2% with RSD 1.1%.Conclusion：This analytical method is of high feasibility with speed and accuracy for measurement and takes advantages in quality control of the opioid alkaloid in rhizome corydalis