三七总皂苷对脑缺血再灌注后细胞外基质破坏及氧化应激的影响

Effect of Panax Notoginseng Saponins on Extracellular Matrix Damage and Oxidative Stress After Cerebral Ischemia-reperfusion in Mice

目的:研究三七总皂苷(PNS)对脑缺血再灌注后细胞外基质破坏及氧化应激的影响,探讨三七总皂苷抗缺血性脑损伤的机制。方法:将C57BL/6小鼠随机分成假手术组、模型组、PNS组及依达拉奉组,连续给药4天后结扎双侧颈总动脉20min,再灌注24h后检测基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)及组织基质金属蛋白酶抑制物-1(tissue inhibitor of meta11oproteinase-1,TIMP-1)在脑组织的表达;脑缺血20min再灌注60min后测定脑组织丙二醛(Malonaldehyde,MDA)、还原型谷胱甘肽(Glutathione,GSH)、一氧化氮(NitricOxide,NO)含量和超氧化物歧化酶(Superoxide Dismutase,SOD)、一氧化氮合酶(Nitric Oxide Synthase,NOS)活性。结果:.脑缺血再灌注后,脑织织MMP-9表达增强,与假手术组比较,差异具有统计学意义(P<0.01),而TIMP-1表达无显著性变化;脑缺血再灌注后脑组织MDA含量显著升高,SOD活性和GSH含量显著降低,NO含量和NOS活性均显著升高,与假手术组比较,差异均具有统计学意义(P<0.01)。PNS可显著升高脑缺血再灌注后TIMP-1蛋白表达,降低MMP-9蛋白表达,与模型组比较,差异均具有统计学意义(P<0.01,P<0.05);PNS可显著降低MDA和NO含量,与模型组比较,差异均具有统计学意义(P<0.01,P<0.05),但对SOD、NOS活性和GSH含量无显著影响。结论:三七总皂苷对脑缺血再灌注后的细胞外基质破坏及氧化应激损伤具有抑制作用。

Objective：To investigate the effect of Panax Notoginseng Saponins（PNS） on extracellular matrix damage and oxidative stress after cerebral ischemia/reperfusion injury in mice.Methods：C57BL/6 mice were randomly divided into sham-operation group,model group,PNS group and Edaravone group.The bilateral common carotid artery of mice were ligated for 20 min followed by 24 hours reperfusion after administration for 4 days,the expression of MMP-9 and TIMP-1 protein in brain tissue was detected,the content of malonaldehyde（MDA）,glutathione（GSH）,nitric oxide（NO） and activity of superoxide dismutase（SOD） and nitric oxide synthase（NOS） in brain tissue after cerebral ischemia for 20 min follwed reperfusion for 60 min was determined.Results：Compared to sham operated group,the expression of MMP-9 protein was in brain tissue in model group incressed markedly（P 〈 0.01）,the expression of TIMP-1 protein had no changes.after cerebral ischemia-reperfusion,The content of MDA,NO and activity of NOS in model group was higher than the sham operated group（P 〈 0.01）,The content of GSH and activity of SOD in model group was signifi-cantly lower than the sham operated group（P 〈 0.01）.The expression of MMP-9 protein,the content of MDA and NO in PNS group was lower than the model group（P 〈 0.05,P 〈 0.01）,the expression of TIMP-1 protein in PNS group was higher than the model group（P 〈 0.01）,the content of GSH and activity of SOD and NOS in PNS group had no changes（P 〉 0.05）.Conclusion：Extracellular matrix damage and oxidative stress injury after cerebral ischemia-reperfu-sion in mice could be inhibted by PNS