摘要
目的探讨酶联免疫吸附试验(ELISA)方法中乙型肝炎病毒表面抗原(HBsAg)弱阳性标本复查方式,确保结果的准确可靠。方法用ELISA法筛查出144例如下的标本:①S/CO值0.8～0.99,14例;②S/CO值在1.0～1.99,60例;③S/CO值在2.0～4.99,38例;④S/CO值在5.0～15,32例,以上标本均进行Elecsys HBsAg定量试验和HBsAg确证试验,对结果进一步确证。结果 144例标本中,三种方法都阳性的标本有62例,Elecsys HBsAg定量试验阳性62例,HBsAg确证试验阳性64例,ELISA与Elecsys HBsAg定量试验,ELISA与HBsAg确证试验比较,均有显著性差异(P<0.05),Elecsys HBsAg定量试验与HBsAg确证试验比较,结果没有显著性差异(P>0.05)。结论 ELISA试验做为临床乙肝的筛查方法容易出现假阳性,弱阳性标本可以用Elecsys HBsAg定量试验进行快速复查。
Objective To discuss the clinical application of methods for rechecking the weak positive resuhs of HBsAg detected by ELISA in serum. Methods We picked up 144 samples by ELISA as follows： （1） S/CO 0.8 -0.99, 14 cases. （2） S/CO 1.0 - 1.99,60 cases. （3） S/CO 2.0 -4.99,38 cases. （4） S/CO 5.0 ~ 15,32 cases,and retestcd the samples by using HBsAg quantitative analysis and HBsAg confirmatory test. Results In 144 samples,62 were positive by using three methods,62 were positive by using HBsAg quantitative analysis ,and 64 were positive by using the confirmatory test. There were significant differences between ELISA and HBsAg quantitative analysis as well as between ELISA and confirmatory test（P 〈0.05 ）,and there was no significant difference between HBsAg quantitative analysis and confirmatory test（P〉0.05 ）. Conclusion It is very common of false positive in HBsAg serum by using ELISA. Therefore,the quantitative analysis and confirmatory test will be used to confirm the results of some weak positive samples rapidly.
出处
《中国现代医生》
2010年第31期84-85,137,共3页
China Modern Doctor
