HPLC测定苦参注射液中苦参碱含量

Content Determination of Matrine in Matrine Injection by HPLC

建立了苦参注射液中苦参碱含量的HPLC检测法。色谱柱为waters C18(4.6 mm×150 mm,5μm),0.02 mol/L乙酸铵缓冲溶液(含500μL/L三乙胺)-甲醇-乙腈(70∶10∶20)为流动相,检测波长210 nm,流速1 mL/min,柱温30℃,进样量20μL。苦参碱进样浓度在4.0～200.0μg/mL范围内与峰面积呈良好的线性关系(r=0.999 9);样品平均回收率为100.1%(n=9),RSD为1.1%。本方法简便、准确度高、重复性好,可用于苦参注射液中苦参碱的质量控制。

An HPLC method for the content determination of matrine in matrine injection was developed.The column was Waters C18（4.6 mm×150 mm,5 μm）,mobile phase was 0.02 mol/L acetate buffer solution（500 μL/L triethylamine）-methanol-acetonitrile（70∶10∶20）,detection wavelength was 210 nm,flow rate was 1 mL/min,column temperature was 30 ℃.The injection volume was 20 μL.There was a good linear correlation between the peak areas and the concentrations of matrine at the range of 4.0～200.0 μg/mL（r=0.999 9）.The average recovery was 100.1%with RSD of 1.1%（n=9）.The results indicated that the method was simple,accurate and repeatable.It can be used for the quality control of matrine in matrine injection.