电针结合重复经颅磁刺激对局灶性脑缺血大鼠内源性神经干细胞迁移的影响

Effects of Electro-acupuncture Combined with rTMS on Migration of Inherent Neural Stem Cells after Focal Cerebral Ischemia in Adult Rats

目的探讨电针结合重复经颅磁刺激(rTMS)对局灶性脑缺血大鼠内源性神经干细胞迁移的影响及其治疗缺血性脑损伤的机制。方法取SD大鼠120只,采用线栓法制备大鼠大脑中动脉闭塞模型,随机分为正常组、模型组、电针组、磁刺激组和结合组,通过免疫组化检测脑缺血后第7、14、28日3个不同时相、不同脑区多唾液酸神经细胞黏附分子(PSA-NCAM)与PSA-NCAM/5-嗅脱氧尿嘧啶(BrdU)表达的变化,并进行神经功能评分。结果缺血后第7日PSA-NCAM和PSA-NCAM/BrdU阳性细胞表达明显增加,第14日达到高峰,第28日表达开始下降;电针组、磁刺激组、结合组与模型组比较在第7、14日时有显著差异,结合组与电针组、磁刺激组比较在第7、14日时有显著差异。电针组、磁刺激组和结合组各时相神经功能评分均较模型组改善,尤以结合组为明显。结论电针结合磁刺激对脑卒中后神经功能的恢复具有显著的促进作用,促进缺血侧不同脑区内源性神经干细胞的迁移可能是其治疗缺血性脑卒中的重要作用机制之一。

Objective： To investigate the effects of electro-acupuncture（EA） combined with repetitive Tran-scranial Magnetic Stimulation（rTMS） on migration of inherent neural stem cells after focal cerebral ischemia in adult rats and to explore the mechanism of EA combined with rTMS in treating ischemic brain injury.Methods： The model of transient focal ischemia was made by the middle cerebral artery occlusion.120 SD rats were randomly divided into normal group,model group,EA group,rTMS group and EA combined with rTMS group.The expressions of poly-siolylated neural cell adhesion molecule（PSA-NCAM） and PSA-NCAM /5-bromodeoxyuridine（BrdU） in dif-ferent encephalic regions of ipsilateral hemispheres were detected by immunohistochemical staining and the neurologic impairment rating was observed at the 7th,14th and 28th day after infarction respectively.Results： The number of PSA-NCAM-positive cells and PSA-NCAM / BrdU positive cells in different encephalic regions of ipsilateral hemispheres increased at day 7 after MCAO,reached maximum at day 14,and decreased markedly at day 28.There were significant differences in EA group,rTMS group and EA ＋ rTMS group when compared with that of model group at day 7 and 14.There were significant differences in EA ＋ rTMS group when compared with that of EA group and rTMS group.The improvement of neural motor function was much better in EA group,rTMS group and EA ＋ rTMS group compared with model group and the improvement were the most obvious in EA ＋ rTMS group.Conclusion： EA combined with rTMS can promote the functional recovery after cerebral arterial thrombosis and enhance the migration of inherent neural stem cells in different encephalic regions of ipsilateral hemispheres,which might be one of the important mechanisms of EA combined with rTMS in treating ischemia brain injury.