利胆排毒口服液抗兔内毒素血症作用的研究

Effect of Lidanpaidu Oral-liquid on Resistance of Rabbit with Endotoxemia

目的观察利胆排毒口服液对家兔内毒素血症的解热作用。方法用大肠杆菌内毒素静脉注射制备家兔内毒素血症模型,观察利胆排毒口服液对模型动物体温变化的影响;用定量检测微量内毒素鲎试验法检测血浆内毒素(LPS)含量,ELISA法检测肿瘤坏死因子(TNF-α)含量。结果 3h体温反应高度及5h体温反应指数在各组间有明显的差异;兔灌服利胆排毒口服液后,可减少血浆LPS含量,降低血浆TNF-α水平。结论利胆排毒口服液对兔内毒素血症有对抗作用。

Objective： To observe the heat-clearing effect of the Lidanpaidu Oral-liquid（LOL） on endotox-emia rabbits.Methods： The endotoxemia model was made by injection with endotoxin of escherichia coli.Some in-dices were observed including the model temperature change,the LPS level and the TNF-α content.The LPS was detected by the tachypleus amebocyte lysate method.And the TNF-α was detected by the ELISA method.Results： In several groups,there were some significant differences between the temperature response height in 3h and the temperature response index in 5h.The LPS level and the TNF-α content were be reduced by the LOL.Conclusion： The endotoxemia in rabbits could be protected by the LOL.