GC/MS法检测汤料中罂粟生物碱提取条件探讨

The extracting condition of Poppy′s alkaloid in soud stock by GC/MS

目的:建立GC/MS检测汤料中罂粟生物碱最佳提取条件。方法:以气质联用中SIM模式进行离子提取,以罂粟壳加入正常的火锅汤底作阳性样品,模拟各种汤底组成中罂粟壳的加入方法,用三种组合的有机溶剂分别作萃取液,定性检测汤料中的可待因、吗啡、蒂巴因、罂粟碱、那可汀等生物碱。结果:在同等的罂粟壳加入量中,加入到无油稀汤底中的罂粟壳生物碱响应值最高,最易检出;用氯仿+乙醇(9+1)作萃取液,罂粟生物碱提取效果最好;以可待因作为检出指标,检测灵敏度最高。结论:以气质联用中SIM模式,用氯仿+乙醇(9+1)作萃取液,选取怀疑添加了罂粟壳的无油稀汤底进行样本检测,罂粟生物碱的检出率最高。

Objective：To develop the best extracting condition of poppy′s alkaloid in soup stock by GC/MS.Methods： To qualitative analyze codeine,morphine,thebaine,paparerine,noscapine in soup stock by extracting selected ions and chromatograph′s RT of poppy′s shell through adding poppy′s shell to the hot pot soup as positive sample,and simulating each kind of soup′s composition of Adding poppy′s shell,making the extracted liquid separately with three kind of combination′s organic solvents.Results： The poppy shell alkaloid response value was the highest in the non-oil thin soup of adding the same quantity of poppy shell.Conclusion： The poppy alkaloid detection rate is highest through selecting that suspected poppy shell′s non-oil thin soup as sample and chloroform ＋ ethyl alcohol（9＋1） as extracting solvent.