湖北咸宁温泉嗜热菌的分离与初步鉴定

Hubei xianling hotsprings thermophilic bacteria separated and to made preliminary identification

目的:分离温泉中的嗜热菌并作初步鉴定。方法:用稀释涂布法分离和纯化嗜热菌并进行形态学观测和生化鉴定。结果:从温泉水中分离获得4株嗜热菌,革兰染色阳性,有芽孢无荚膜和鞭毛,细胞呈杆状,培养特性为pH值(6～7),最适生长温度55℃～60℃。结论:初步鉴定为嗜热脂肪芽孢杆菌。

Objective：To hotsprings thermophilic bacteria separated and to made preliminary identification.Methods： Use with the dilution separation methods purified thermophilic bacteria,carry through morphology observation and biochemistry identified.Results： From the hotspring water separated four thermophilic bacteria,Gram stain positive,have spore,no capsule and flagellum,cell assumed assumed pole state,culture characteristics was pH value（6～7）,optimum growth temperature 55℃～60℃.Conclusion： Early step identified as thermophilic fat spore bacillus.