儿童呼吸道合胞病毒感染检测分析

Analysis on respiratory syncytial virus in children infection

目的:对深圳地区儿童呼吸道合胞病毒进行检测,探讨其流行规律。方法:收集深圳市第四人民医院毛细支气管炎、支气管肺炎等患儿标本,RT-PCR检测标本中的呼吸道合胞病毒,与病毒抗原免疫荧光检测方法进行对照。结果:686例标本中,呼吸道感染患儿中RSV感染达到16.5%(113/686),本方法与免疫学检测方法具有较高的相关性和符合率,均可用于呼吸道合胞病毒的早期诊断。结论:呼吸道合胞病毒是深圳地区儿童呼吸道感染的重要病毒病原,RT-PCR检测方法可用于儿童呼吸道合胞病毒感染的临床诊断。

Objective：To establish the method results to detect respiratory syncytial virus（RSV） by molecular biology,and to understand the distribution of the pathogen.Methods： RSV were identified from nasopharyngeal aspirates and throat swabs collected from children with bronchiolitis,bronchopneumonia who visited the Pediatrics of Shenzhen Fourth People′s Hospital.The RSV was detected by RT-PCR.Clinical samples were detected,and the results were compared to immune assay.Results： RSV were detected in 686 patients,the frequent is 16.5% of 113 patients.The correlation of the molecular biology method is similar to immune assay.Conclusion： The data indicate that RSV is an important etiological agent for respiratory infections in children during the survey period.The detecting method by RT-PCR can be applied to the clinical diagnose of the RSV infection.