湖州市食品中副溶血性弧菌污染状况、血清分型及耐药性监测

Analysis on contamination status,serotype and drug resistance of Vibrio parahaemolyticus isolated from food in Huzhou city

目的:了解湖州市食品中副溶血性弧菌的耐药性、血清型和污染状况,初步确定可能污染副溶血性弧菌的高危食品,为防止副溶血性弧菌引起的食源性疾病提供科学依据。方法:采集农贸市场、超市等各类食品,按照GB/T4789-2003方法进行分离鉴定,用API20E试剂条做进一步生化鉴定。用标准血清进行分型,用纸片扩散法进行药敏试验。结果:检测生禽肉、生畜肉、生食水产品、生食蔬菜、熟肉制品、鲜冻水产品及蔬菜沙拉共127件样品,检出副溶血性弧菌17份,检出率为13.39%(17/127),其中生禽肉中检出率最高29.4%。分离的17株副溶血性弧菌属于7个血清群,分别为0:5群占47.06%(8/17)、O:6群占23.53%(4/17)、0:1群、O:2群、O:4群、O:8群、O:11群各占5.89%(1/17)。分离菌株对诺氟沙星、头孢噻肟、环丙沙星、奈啶酸、四环素、复方新诺明、氯霉素、阿米卡星和庆大霉素100%敏感,对头孢噻吩的耐药率达到58.8%。所有菌株对氨苄西林及阿莫西林全部耐药,1株对四环素耐药。结论:湖州市食品中副溶血性弧菌污染率较高,存在安全隐患。同时要防止由耐药菌株引起的爆发流行。

Objective：To understand the contamination status,serotype and drug resistance of Vibrio parahaemolyticus（VP） isolated from food and provide scientific evidence for prevention of foodborne diseases caused by VP.Methods： According to GB/T4789-2003,VP were isolated from different kinds of food samples collected from market and identificated by API 20E system for further biochemical appraisal.Antibiotic susceptibility of these VP isolates was performed with Agar diffusion method and the serotype was determined by slide agglutination test.Results： A total of 17 strains of VP were isolated from 127 food samples with the detection rate of 13.39%（17/127）.The detection rate of VP in poultry was the highest.,which was 29.4%.The 17 VP isolates were serotyped into 7 serum groups： 0： 5 groups accounted for 47.06%（8/17）;O：6 groups accounted for 23.53%（4/17）;0： 1 group,O： 2 groups,O： 4 group,O： 8 groups,O： 11 groups each accounted for 5.89%（1/17）.The sensitive rate of the 17 isolates to norfloxacin,cefotaxime,ciprofloxacin,nalidixic acid,tetracycline,cotrimoxazole,chloromycetin,Amikaxi and gentamicin was 100%,while the drug resistance rate to Cephalothin was 58.8%.All of the stains were resistance to ampicillin and amoxicillin.Conclusion： We should pay more attention to the high contamination rate of VP in food which may cause some potential safety problems and prevent the out-break caused by drug-resistant strains.