嘉兴市新发现HIV感染者CD4^+ T淋巴细胞的检测分析

First detection and analysis of CD4^+ T-lymphocytes for new-found HIV infected persons in Jiaxing

目的:通过对嘉兴地区新发现的155例HIV-1抗体阳性的感染者,进行首次的CD4+ T淋巴细胞绝对值等的测定,来了解并估计其的免疫状况及疾病的进展状况。方法:对采集的155份全血样本以四色荧光抗体进行标记,通过流式细胞仪进行CD4+和CD8+ T淋巴细胞绝对值的检测,并计算CD4+/CD8+的比值。统计分析其测定值。结果:嘉兴市能随访到的HIV阳性感染者并完成CD4+ T检测的共计155例,其中CD4+ T淋巴细胞平均为296.68±212.88个/μl,CD4+/CD8+比值平均为0.37±0.24,已有36.8%HIV感染者CD4+ T<200个/μl,CD4+/CD8+<1的占98.1%;不同性别和3种传播途径的CD4+ T淋巴细胞和CD4+/CD8+比值,在结果上无显著性差异;2个年龄组间CD4+ T淋巴细胞有显著性差异,高年龄组的数值要低于低年龄组的。结论:嘉兴市有1/3新发现的HIV感染者已进入艾滋病期,应对其定期检测,估计疾病进展和及时药物治疗;同时要进行更多的艾滋病防治宣传,鼓励更多高危人群进行相关HIV抗体和CD4+ T淋巴细胞的检测,以把握治疗的最佳时期。

Objective：To first detect 155 new-found HIV infected persons about the CD4＋ T-lymphocyte Count（CD4＋ T-LC）,in order to better learn their immune status and development of diseases.Methods： 155 samples of whole blood were tested by flow cytometry with 4-color fluorescence labeling antibodies,and analyzed by CD4＋ T-LC,CD8＋ T-LC and the rate of both（CD4＋/CD8＋）.Results： The mean value of CD4＋ T-LC and CD4＋/CD8＋ rate are 296.68±212.88/μl and 0.37±0.24 respectively,and the persons less than 200/μl（CD4＋ T-LC） and 1（CD4＋/CD8＋ rate） account for 36.8% and 98.1% of tested HIV infected persons.The values between genders and transmission routes are not different obviously,but in two age groups,the values in high age group are lower.Conclusion： In Jiaxing,there are one third of new-found HIV infected persons in AIDS period.It′s necessary for the HIV infected persons and high risk population to be encouraged to accept the relative tests,HIV antibodies and CD4＋ T-lymphocytes,so as to grasp the best period of treatment.