壳聚糖包裹紫杉醇纳米粒子的制备及性能研究

Preparation of Paclitaxel-loaded chitosan nanoparticles and its physicochemical characterization

目的探讨壳聚糖-紫杉醇纳米粒子(CS-PTX-NP)的制备方法并研究其特性。方法采用溶剂挥发-乳化交联法制备壳聚糖-紫杉醇载药纳米粒子,借助红外光谱仪分析粒子结构,使用透射电子显微镜检测粒子的形态、粒径及分布,紫外分光光度计测定载药量和包封率,并考察粒子的体外释放性能,探讨影响制备该粒子的主要因素。结果粒子的平均粒径为(116±3.07)nm,成球性、分散性好,粒子的包封率最大可达94.04%,主要受Mptx∶Mcs、搅拌速度的影响。粒子在生理条件下具有明显的缓释作用。结论壳聚糖紫杉醇纳米粒子的制备为开发脂溶性药物缓释制剂提供了一条新的途径。

Objective To Prepare chitosan-paclitaxel nanoparticles and study their characteristics.Methods Taxol and Chitosan-loaded nanoparticles were prepared by solvent evaporation and emulsion cross-linking.The structure of particles was analyzed by infrared spectroscopy;and the size,morphology and distribution of particles were examined by transmission electron microscope.Finally the drug-loading and encapsulation rate as well as its in vitro release characteristics were all analyzed by UV-spectrophotometer.Results The average diameter of nanoparticles was 116±3.07nm with good encapsulation efficiency up to 94.04% which were mainly influenced by mptx/mcs ratio and the stirring speed.Sustained release under physiological conditions was observed in these particles.Conclusion Nanoparticles containing paclitaxel shelled with chitosan may provide a new approach for the development of fat-soluble drug delivery.