白念珠菌SSK1突变株对氟康唑敏感性的初步研究

Antifungal activity of fluconazole against Ssk1p mutant of Candida albicans

目的探讨氟康唑作用于白念珠菌双组分信号传导途径SSK1突变株SSK21后药物敏感性的变化。方法采用微量液体稀释法和固醇测定法测定野生株(CAF2-1)和突变株(SSK21)的最小抑菌浓度(MIC);并应用RT-PCR观察氟康唑作用前后,SSK1的表达变化。结果氟康唑对CAF2-1的MIC为16μg/mL,对SSK21为0.032μg/mL。加入氟康唑后,CAF2-1的SSK1表达明显增加,60min时达到最多。结论 SSK21对氟康唑高度敏感,SSK1基因及其相关的重要基因与药物敏感性的关系值得进一步研究,从而为新的抗真菌药物和治疗途径的研发提供参考。

Objective To investigate the in vitro antifungal activity of fluconazole against two-component signal transduction protein Ssk1p mutant of Candida albicans （SSK21）.Methods The minimum inhibitory concentrations （MIC） of fluconazole against SSK21 and wild type of C.albicans （CAF2-1） were tested by broth microdilution and ergosterol content assays.RT-PCR was performed to determine the expression of SSK1 in CAF2-1 in the presence of fluconazole.Results The MIC of fluconazole against SSK21 was 0.032 μg/mL,while that against CAF2-1 was 16 μg/mL.After administration with fluconazole,the transcription level of SSK1 from CAF2-1 was increased.Conclusions SSK21 is hypersensitive to fluconazole compared with wild type strain CAF2-1.Ssk1p and related genes should be exploited for new therapeutic approach.