MAGE-1基因在肝细胞性肝癌中的表达

Expression of MAGE-1 gene in hepatocellular carcinoma

目的探讨ＭＡＧＥ－１基因编码的肿瘤相关抗原作为肝细胞性肝癌（ＨＣＣ）主动免疫治疗攻击靶点的可能性。方法用ＲＴ－ＰＣＲ的方法检测了３９例ＨＣＣ及癌旁组织、３种人肝癌细胞株和５例非ＨＣＣ病例肝组织中ＭＡＧＥ－１基因的表达。结果ＨＣＣ癌组织中ＭＡＧＥ－１基因表达率明显高于癌分组织中的表达率，５８．９％（２／３９）对１０．３％（４／３９），Ｐ＜００１。３种细胞株均为阳性表达。结论ＭＡＧＥ－１基因可作为主动免疫治疗的攻击靶点，以进一步研究设计新的ＨＣＣ免疫治疗策略。

Objective To explore the possibility of tumor associated antigen encoded by MAGE-1gene used as a tarest for immunotherapy in HCC patients. Medthod The expression of MAGE-1 gene in39 HCCs and 3 HCC Cell lines and 5 non-HCC patients liver tissues was examined by RT--PCR method.Besults The expredon rate of MAGE-1 mRNA was singnificantly higher in HCC patients than inparatumor tissues (P<0.01, 58.9% vs 10.3%). All the 3 HCC cell lines expressed MAGE-1 gene positively.All 5 non-HCC patients' liver tissues did not express MAGE-1 gene. Conclusion The high frequency ofexpression of MAGE-1 gene in HCCs suggests the possibility of MAGE-1 used as a target for immuno- therapy in HCC patients.