非酒精性脂肪肝肝细胞色素P450ⅡE1的表达与氧化抗氧化的关系

Correlation between hepatocyte cytochrome P450IIE1 expression and oxidation, antioheation in rat nonal-coholic steatosis model

目的研究非酒精性脂肪肝肝细胞色素Ｐ４５０ⅡＥ１表达及与氧化抗氧化的关系。方法用免疫组织化学的方法研究高脂饲料诱导的大鼠脂肪肝肝细胞色素Ｐ４５０ⅡＥ１的表达，同时检测肝组织中雨二醛（ＭＤＡ）和超氧化物歧化酶（ＳＯＤ）、谷胱甘肽（ＧＳＨ）、维生素Ｅ（ＶｉｔＥ）含量的变化，并分析其相关性。结果高脂饲料诱导的大鼠脂肪肝肝细胞色素Ｐ４５０ⅡＥ１在腺泡３区的表达增强，并从３区弥散至２区，与正常组相比，脂肪肝肝组织中ＭＤＡ的含量显著升高（Ｐ＜０．０１）．而ＳＯＤ、ＧＳＨ、ＶｉｔＥ的含量显著下降（Ｐ值分别小于０．０１、０．０５、０．０１）。相关分析表明，肝ＣＹＰＨＥＩ的表达与ＭＤＡ呈非常显著的正相关（Ｐ＜０．０１）、而与ＳＯＤ、ＧＳＨ、ＶｉｔＥ呈显著的负相关ｕ值分别小于０．０５、０．０５、０．０１）。结论非酒精性脂肪肝肝细胞色素Ｐ４５０ⅡＥ１在腺泡３区的表达增强，并呈弥散性的分布，与酒精性脂肪肝的表达相似，并可能与脂质过氧化反应有关。

Objective To study the correlation between hepatocyte cytochrome P45ⅡEI(Cyp Ⅱ E1)expression and oxidation, antioxidation in rat nonalcoholic steatosis model. Methods The expression ofhepatocyte CYPⅡE1 antigen in rat steatosis model induced by high fat diet was detected withimmnohistochemistry. Meanwhile, MDA, SOD, GSH and VitE contents in the liver were measured bybiochemical analysis. Results Expression of CYPⅡE1 in rat nonalcoholic steatosis by high fat diet wasprominent in hepatic acinar zone 3, and had a more extensive acinar distribution from zone 3 to zone 2,MDA contents in fatty liver was significantly increased than in normal, SOD, GSH and VitE contentswere significantly deecreased than in normal. There were significant positive correlation between CYPⅡE1and MDA (p<0.01). and negstive correlation between CYPⅡE1 and SOD, GSH, VitE (P<0.05, P<0.05, P<0.01). conclusions Hepatocyte CYP ⅡE1 expression in nonalcoholic steatosis is prominent in hepatic aci- nar zone 3. Its expression resembles alcoholic fatty liver. There is significant correlation between CYPⅡE1 and lipid peroxidation.