转化生长因子β1基因-509位点多态性与重度慢性牙周炎易感性的关系

Relationship between transforming growth factor beta-1 gene-509C/T polymorphism and severe chronic periodontitis

目的 探讨转化生长因子β1（transforming growth factor beta-1,TGF-β1）基因-509位点多态性与重度慢性牙周炎易感性的关系,以期从基因水平探讨牙周炎发病的遗传学机制.方法 用聚合酶链反应-限制性片段长度多态性方法检测102例重度慢性牙周炎患者（牙周炎组）和102名健康对照者（健康对照组）的TGF-β1基因-509位点,比较两组间此位点基因型分布和等位基因频率的差异.结果 TGF-β1基因-509位点CC、CT、TT基因型在牙周炎组和健康对照组的分布频率分别为44.1%（45/102）、47.1%（48/102）、8.8%（9/102）和29.4%（30/102）、51.0%（52/102）、19.6%（20/102）,两组人群基因型分布频率差异有统计学意义（P＜0.05） 等位基因C、T在牙周炎组和健康对照组分布频率分别为67.6%（138/204）、32.4%（66/204）和54.9%（112/204）、45.1%（92/204）,两组人群的等位基因分布频率差异亦有统计学意义（P＜0.05）,C等位基因携带者患重度慢性牙周炎的风险是T等位基因的1.718倍（OR=1.718,95%CI：1.148～2.569）.结论 TGF-β1基因-509位点多态性与重度慢性牙周炎的发病具有相关性,C等位基因可能是重度慢性牙周炎的遗传易感基因.

Objective To investigate the relationship between transforming growth factor beta-1 （TGF-β1 ）gene-509C/T polymorphism and severe chronic periodontitis in Chinese Hans population.Methods TGF-β1-509C/T genotype polymorphism was analyzed in 102 patients with severe chronic periodontitis（periodontitis group） and 102 healthy controls（ control group） by polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） method.Results The distributions of TGF-β1-509C/T genotype and allele were significantly different between severe chronic periodontitis group and control group （ P 〈 0. 05 ）. TGF-β1-509CC, CT and TT genotype frequency were 44. 1% （ 45/102 ）, 47. 1%（48/102） ,8. 8% （ 9/102 ） in periodontitis group and 29. 4% （ 30/102 ）, 51.0% （ 52/102 ）, 19. 6%（20/102） in control group, respectively. The relative risk analysis found that C allele carriers had higher risk of suffering from severe chronic periodontitis compared with T allele carriers（ OR = 1. 718,95% CI：1. 148 ～ 2. 569 ）.Conclusions TGF-β1-509C/T polymorphism is associated with severe chronic periodontitis in Chinese Hans population, and C allele may be an important genetic susceptibility gene for severe chronic periodontitis.