口腔鳞状细胞癌中整联蛋白连接激酶的表达及意义

Expression of integrin-linked kinase in oral squamous cell cancinoma and its significance

目的 研究整联蛋白连接激酶（integrin-linked kinase,ILK）在口腔鳞状细胞癌中的表达特点,以期进一步揭示其与口腔鳞状细胞癌的生物学行为的关联.方法 采用免疫组织化学及组织化学方法研究ILK在170例口腔鳞状细胞癌中的表达情况及规律.结果 ILK的阳性表达可以出现在癌巢或癌细胞条索内和（或）癌组织外围的间质.随着口腔鳞状细胞癌病理分级的提高,ILK具有从阴性表达、仅在上皮表达、上皮间质均表达发展为仅在间质表达的趋势.经非参数检验,无论在无转移组[高、中、低分化组ILK仅在间质表达者分别为2%（1/50）、18%（6/33）和64%（18/28）]和转移组[高、中、低分化组仅在间质表达者分别为16%（4/25）、9/18和10/16],ILK的表达模式与病理分级间差异均有统计学意义（F=17.742,P＜0.001 F=4.394,P=0.017）.ILK的这种表达模式在有、无淋巴结转移组间的差异也具有统计学意义（x2=14.418,P=0.002）.结论 在口腔鳞状细胞癌的发展过程中ILK更趋向表达于间质,其本质值得深入研究.

Objective To investigate the expression patterns of integrin-linked kinase （ILK） in oral squamous cell carcinoma（OSCC）. MethodsThe expression of ILK in 170 OSCC cases was examined by immunohistochemistry and periodic acid-schiff histochemistry. ResultsILK immunoreactivity occurred in cancer cells and（or） stroma encircling the malignant tissue. Along with the deteriorated histopathologic grade of oral cancer, there was stepwise aberration exhibiting from ILK-negative, to ILK-positive in epithelia, to ILK-positive in both epithelia and stroma, and to ILK-positive in stroma.Through nonparameter test, in both lymphatic invasion [ILK-positive in stroma in well-differentiated、 moderatelydifferentiated and poorly-differentiated is 2% （1/50）, 18% （6/33） and 64% （18/28）, respectively] and non-lymphatic invasion group [ILK-positive in stroma in well-differentiated、 moderately-differentiated and poorly-differentiated is 16% （4/25）, 9/18 and 10/16, respectively）, the expression patterns of ILK exhibited significant correlations with histopathology （F = 17. 742, P 〈0. 001 ,F =4. 394, P =0. 017）. ILK expression between lymphatic metastasis and non-lymphatic metastasis group was significantly different （x2 = 14. 418, P =0. 002）. ConclusionsILK inclines to express in stroma during the OSCC progress.