大七气汤对大鼠异位子宫内膜VEGF、MMP-9表达的影响

Effect of Traditional Chinese Medicine (Daqiqi Tang) on Expresstions of VEGF and MMP-9 in Endometriosis Rats

目的:探讨中药大七气汤治疗子宫内膜异位症的机理。方法:建立SD大鼠子宫内膜异位模型,成功建模3周后将大鼠随机分模型组(B组,n=8)、大七气汤大剂量组(40 g/kg,C组,n=10)、小剂量组(20 g/kg,D组,n=8)、孕三烯酮组(0.5 mg/kg,E组,n=8),并以假手术为正常对照组(A组,n=8),每组给药3周,然后用免疫组织化学方法检测在位、异位子宫内膜组织血管内皮生长因子(VEGF)、金属蛋白酶(MMP)-9的表达情况。结果:C、D、E组均降低异位内膜组织VEGF/MMP-9的表达,与B组比较差异均有统计学意义(P<0.05);C、D、E组间无明显差异(P>0.05)。结论:大七气汤能明显抑制子宫内膜异位症模型大鼠异位内膜VEGF、MMP-9的表达,从而明显抑制异位内膜周围血管的形成,使异位内膜萎缩。

Objective： To investigate the mechanism of the Traditional Chinese Medicine（TCM）——Daqiqi Tang that have some positive treatment to endometriosis.Methods： The endometriosis model was established in SD rats and the model rats were divided into 4 groups that were model group（group B,n=8）,TCM high-dose Daqiqi Tang 40 mg/kg（group C,n=10）,TCM low-dose Daqiqi Tang 20 mg/kg（group D,n=8）,gestrinone group（0.5 mg/kg,group E,n=8）,while sham operiation as control group（group A,n=8）.After 3 weeks of treatment,ectopic endometrial grafts were examined for the volume and histomorphology.The expression of VEGF was observed by immunohistochemistry（SABC technique） and MMP-9 was determined by immunostaining.Results： Both VEGF and MMP-9 protein expressions were decreased,and was statistically significant（P0.05）.However,there was not obvious difference among C,D and E group.Conclusion： DaqiqiTang can inhibit the VEGF and MMP-9 protein expression in the ectopic endometrium of rat,suppress the vascularizaton in ectopic endometrium and results in atrophy of endometrium eventually.