晚期糖基化终产物受体、P-选择素与子痫前期的相关性研究

Correlation between Glycation End Product Receptor,P-Selectin and Preeclampsia

目的:探讨晚期糖基化终产物受体(receptor for advanced glycation end products,RAGE)、P-选择素(P-selectin)在子痫前期发生、发展中的作用及意义。方法:应用RT-PCR检测30例正常孕妇和40例子痫前期患者(轻度子痫前期患者20例和重度子痫前期患者20例)胎盘组织和血液中RAGE和P-选择素mRNA的表达情况。结果:RAGE和P-选择素在子痫前期患者和正常孕妇胎盘组织和血液中均有表达。子痫前期组RAGE、P-选择素的mRNA表达水平均高于正常孕妇(对照组)(P<0.05),且重度子痫前期组高于轻度子痫前期组(P<0.05)。RAGE和P-选择素在子痫前期胎盘组织和母血中的表达均成正相关(胎盘r=0.814,血液r=0.796)。结论:子痫前期患者胎盘组织及血液中RAGE、P-选择素的mRNA表达升高可能与子痫前期的发病及病情发展有关;RAGE、P-选择素在子痫前期的发生、发展中可能有协同作用。

Objective： To explore the role and significance of glycation end product receptor（RAGE） and P-selectin in the development of preeclampsia.Methods： RT-PCR analysis was used to detect the expression of RAGE and P-selectin mRNA in placenta tissue and maternal blood from 40 cases of preeclampsia（20 cases of mild preeclampsia and 20 cases of severe preeclampsia） and 30 cases of normal term pregnant.Results： Expression of RAGE and P-selectin mRNA in placenta tissue and maternal blood was both detected in preeclampsia and normal group.Compared with normal group,the levels of RAGE and P-selectin in placenta tissue and serum of preeclampsia were elevated（P0.05）,and the expression levels of severe preeclampsia were higher than those in mild preec-lampsia（P0.05）.The expressions of RAGE and P-selectin in placenta tissue and serum were all positively related.Conclusion： The high levels of RAGE and P-selectin in placenta tiusse and maternal blood may be associated with the occurrence and development of preeclampsia,and both of them may have synergies role in the development of preeclampsia.