高效液相色谱法测定人血浆中艾咪朵尔与血浆蛋白的结合率

Determination of plasma protein binding rate of imidol in human plasma by HPLC

目的建立测定艾咪朵尔血浆药物浓度的HPLC法,并考察艾咪朵尔的人血浆蛋白结合率。方法采用平衡透析法并结合HPLC法,对艾咪朵尔的人血浆蛋白结合率进行测定。结果低、中、高3个浓度下,艾咪朵尔的血浆蛋白结合率分别为89.3%、88.4%和88.1%。结论本方法灵敏度高,重现性好,操作简单,能够满足分析要求。艾咪朵尔与血浆蛋白具有较强的结合。

AIM To develop a HPLC method for the determination of imidol in human plasma and to study the plasma protein binding rate of imidol.METHODS The equilibrium dialysis combined with HPLC to determine the plasma concentration and plasma protein binding rate of imidol was carried out.RESULTS The plasma protein binding rates of imidol at three concentrations were 89.3%,88.4% and 88.1%,respectively.CONCLUSION The HPLC method is high sensitivity,good reproduction,simple management and fulfilling the requirement of analysis.The binding of imidol to plasma protein is of high strength.