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腺病毒介导的IL-24表达对PC-3人前列腺癌细胞体内外抑癌效应 被引量：2

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摘要 目的:研究腺病毒介导的IL-24基因表达对前列腺癌细胞体内外的抑癌效应及分子机制。方法:将扩增的Ad-IL-24感染PC-3细胞,用RT-PCR和Western blot法检测IL-24基因在PC-3细胞中的表达;MTT法检测IL-24基因表达对PC-3细胞的生长影响;流式细胞术(FCM)检测细胞凋亡变化;RT-PCR法检测IL-24基因的表达对PC-3细胞中的Bcl-2、Bax、p53和Caspase3凋亡相关基因表达的影响。用Ad-IL-24在裸鼠PC-3移植瘤的瘤体内注射治疗,通过免疫组化法检测Bcl-2、Bax、Caspase-3、CD34等与细胞凋亡和血管形成相关因子的表达。结果:IL-24基因在PC-3细胞中成功表达,对PC-3细胞增殖有明显抑制作用,可上凋p53、Bax、Caspase-3基因和下凋Bcl-2基因表达,进而诱导细胞凋亡。Ad-IL-24能显著抑制裸鼠前列腺癌移植瘤生长,瘤重的抑制率可达54%;免疫组化结果显示Ad-IL-24不仅能明显上调Bax和Caspase-3基因和下调Bcl-2基因表达,而且能引起与血管形成标记基因CD34表达水平下降。结论:腺病毒介导的IL-24基因表达在体内外可明显抑制PC-3细胞的生长,诱导其凋亡。其机制可能与上凋P53、Bax、Caspase-3基因和下凋Bcl-2和CD34基因表达水平有关。
作者 张治国 杨吉成 杨慧翠 盛伟华 谢宇锋 龙建华 丁翔 欧阳骏
机构地区 苏州大学附属第一医院泌尿外科 苏州大学医学部细胞与分子生物学教研室
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2010年第11期1085-1088,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 江苏省医学重点人才项目(RC2007080) 江苏省卫生厅科研课题(H200610)
关键词 IL-24基因 腺病毒载体 前列腺癌 肿瘤抑制
分类号 R392.11 [医药卫生—免疫学]
  • 相关文献

参考文献10

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二级参考文献26

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共引文献9

同被引文献26

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引证文献2

二级引证文献8

细胞与分子免疫学杂志
2010年 第11期

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