他巴唑和甲状腺素对Graves’病患者sIL-2R的影响

The effect of methimazole and thyroxine on soluble interleukin-2 receptor in patients with Graves' dcisease.

目的研究他巴娃和甲状腺素对Ｇｒａｖｅｓ’病患者ｓＩＬ－２Ｒ的影响。方法将２５例Ｇｒａｖｅｓ’病（ＧＤ）患者外周血单个核细胞（ＰＢＭＣ）在不同浓度的他巴唑（ＭＭＩ）和甲状腺素（Ｔ４）条件下进行体外培养，同时检测培养上清液及血浆ｓＩＬ－２Ｒ水平并与血浆游离Ｔ２（ＦＴ３）和游离Ｔ４（ＦＴ４）作相关分析。结果ＧＤ患者血浆ｓＩＬ－２Ｒ较正常对照明显升高（Ｐ＜０．０１），并与血浆ＦＴ３和ＦＴ４成正相关（分别为（Ｐ＜０．０５和Ｐ＜０．０１）；ＧＤ患者ＰＢＭＣ培养上清ｓＩＬ－２Ｒ较正常对照明显升高（Ｐ＜０．０１），１０－５Ｍ的ＭＭＩ可降低ｓＩＬ－２Ｒ水平（Ｐ＜０．０１），Ｔ４体外对ｓＩＬ－２Ｒ水平无明显影响（Ｐ＞０．０５）。结论ＧＤ患者存在免疫功能异常，ＭＭＩ可调节ＧＤ患者免疫异常；Ｔ４体外对ＧＤ患者ＰＢＭＣ释放ｓＩＬ－２Ｒ无影响。

Obiective To investigate the effect of methimazole and thyroxine on sIL--ZR inpatients with Graves' disease. Methods Peripheral blood mononuclear cells (PBMC) isolated from25 Graves' desease (GD) patients were incubated with or without different concentrations of methimazole (MMI) and thyroxine (T4). The supernatant of culture was used for detecting soluble interleukine 2 receptor (sIL-2R). In the mean time the levels of sIL-2R in Patients' plasma were also detected. All the data were treated statistically by students test and analysis of linear correlation. Results The level of sIL-2R in GD patients 'was significantly higher than that in normal individualgroup and interrelated significantly with FT3 and FT4 (P<0. 05 and P<0. 01 respectively). Thelevel of sIL-2R in supernatant were significantly higher and MMI can decrease the level of sIL-2Rat 10u5M concentration (P<0. 01). Thyroxine had no effect on sIL- 2R in supernatant of GD patients (P>0.05). Conclusion There is immune function abnormality in GD patients and MMI mayturn this abnormality to relatively normality. But thyroxine has no effect on sIL-ZR secretion ofPBMC from GD patients in vitro.