高效液相色谱法测定胃炎康胶囊中桂皮醛的含量

Determination of cinnamaldehyde in weiyankang capsule by HPLC

目的建立胃炎康胶囊中桂皮醛的测定方法。方法样品用50%甲醇超声提取,采用二极管阵列检测器检测,使用XDB-C18柱,流动相为乙腈-0.033 mol·L-1磷酸二氢钾(45∶55),流速1.0 ml·min-1,检测波长290 nm。结果桂皮醛在0.995～9.95 g·L-1(r=0.999 9)内线性良好,平均回收率为101.71%,RSD=1.67%。结论本方法操作简便、灵敏、准确,可用于该制剂的质量控制。

Aim To establish a method for the determination of cinnamaldehyde in weiyankang capsule.Methods The specimen was extracted with 50% methanol ultrasound extraction.HPLC-DAD analysis was carried out on XDB-C18 column with acetonitrile-0.033 mol·L-1potassium dihydrogen phosphate solution（45∶55） as the mobile phase.The detection wavelength was 299 nm and the flow rate was 1.0 ml·min-1.Results The linear range of cinnamaldehyde was 0.995～9.95 mg·L-1（r=0.999 9） with average rate of recovery 101.71%,RSD was 1.67%.Conclusion The method is simple,accurate and reliable,which can be a quality control standard of weiyankang capsule.