不同直径家蚕丝素纳米纤维对星形胶质细胞生长与迁移的作用

The Role of Silk Fibroins with Different Diameters on Astrocytes

目的通过研究3种不同直径的家蚕丝素纳米纤维对纯化的星形胶质细胞生长发育的影响,寻找理想的家蚕丝素纳米纤维的物理参数,为后期使用再生丝素纤维改变神经损伤后形成的物理屏障和化学屏障提供参考和借鉴。方法将混合培养纯化的星形胶质细胞接种在3种不同直径的家蚕丝素纳米纤维材料上,分别选取各时间段的细胞进行星形胶质酸性蛋白(GFAP)特异性免疫荧光染色和活细胞拍摄,并统计不同时间段星形胶质细胞的增殖、铺展和迁移状况。结果第6d时400nm材料上星形胶质细胞的增殖和铺展面积与1200nm相比差异有统计学意义(P<0.05),但是3种直径的材料对星形胶质细胞铺展的最大长度没有显著作用。800nm和1200nm的材料在细胞增殖、铺展面积和最大长度上的差异都不大。结论家蚕丝素纳米材料的直径能影响星形胶质细胞的增殖和铺展,尤其是直径为400nm的材料。

Objective By culturing astrocytes on the regenerated silk fibroin to detect the possibility of the application of the silk fibers on the regeneration of central nervous system. Methods Astrocytes were purified through the repeated passage. We applied different diameters of silk nanofibers as the cells substrates and analyzed the attachment,proliferation and migration of glial cells on these materials. Results Cell proliferation and spreading area were apparent on the 400 nm substrate,being significantly greater than that on 1200 nm substrate on the 6th day（ P 0. 05） . There was no difference in the longest length on the three different diameters of materials. Conclusion The silk fibroin films with small diameters are more suitable for the spreading and proliferation of glial cells than substrates with big diameters substrates.