巢式PCR法检测日本血吸虫低感染度宿主血清DNA的研究

DNA Detection of Low-intensity Schistosoma japonicum Infection by Nested-PCR Assay in Serum of Host

目的探讨日本血吸虫低感染度宿主血清的DNA检测方法,为血吸虫病的早期诊断及疗效考核提供实验依据。方法建立并优化巢式PCR反应体系用于检测不同感染度家兔血清中的日本血吸虫特异性基因片段。结果在仅存活3～5对成虫的感染后家兔血清中均能扩增到特异性目的条带,尤其是感染后3d即可检出。结论巢式PCR可用于日本血吸虫低感染度宿主血清DNA检测,具有潜在应用前景。

Objective To study the DNA detection of low-intensity Schistosoma japonicum infection in serum of host for early diagnosis and therapy evaluation of Schistosomiasis. Methods A nested-PCR assay was established and optimized to detect the S. japonicum specific DNA from the sera of rabbits during 3d to 19w post-infection. Results After infection,a S. japonicum specific DNA fragments were obtained from all the sera samples of rabbits in which 3 ～ 5 pairs of worms lived,especially,that were detected at the third day after infection. Conclusion The nested-PCR is a potential tool in diagnosis of low-intensity S. japonicum infection.