Stathmin mRNA在树突状细胞中的表达及其意义

Expression of Stathmin mRNA in Dendritic Cells and Its Significance

目的探讨StathminmRNA在树突状细胞中的表达及其意义。方法分离和培养小鼠的树突状细胞,用细菌脂多糖(LPS)刺激0h、3h、12h后,收集细胞,并提取细胞总RNA,经RT-PCR反转录为cDNA,再通过荧光定量实时PCR检测Stathmin的mRNA水平。结果定量PCR标准曲线的相关系数为-0.99,且融解曲线峰单一,表明已成功建立了准确、特异的实时定量PCR方法。经LPS刺激后,树突状细胞Stathmin的mRNA表达水平降低,随时间的延长而递减(P<0.01)。结论树突状细胞的Toll样受体4信号途径与Stathmin的表达相关。

Objective To study the expression level of Stathmin mRNA in dendritic cells（ DCs） and its significance. Methods DCs were generated from bone marrow cells of mice,the cells were harvested after incubating with or without LPS. The total RNA was extracted from DCs and then transcribed reversely to cDNA. The expression levels of Stathmin mRNA in DC were examined by real-time PCR. Results The results showed that the correlation coeffecient of the standard curve was-0. 99,and the melting curve showed a single peak,proving that an accurate real-time PCR methed was successful established. Realtime PCR showed that the mRNA levels of Stathmin in DC was downregulated after LPS stimulation（ P 0. 01） . Conclusion TLR4 pathway is involved in the Stathmin expression in dendritic cells.