4NQO诱发C57BL/6小鼠舌癌及食管鳞状细胞癌模型的建立

C57BL/6 Mice Model of Tongue Cancer and Esophageal Squamous Cell Carcinoma Induced by 4-nitroquineline-1-oxide

目的为探讨化学诱导舌癌和食管癌的作用及可能机制,利用化学致癌剂4硝基喹啉1氧化物(4NQO)建立C57BL/6小鼠的舌癌和食管癌模型。方法 100μg/ml、50μg/ml、10μg/ml的4NQO通过饮水法作用于C57BL/6小鼠,实验期间通过肉眼和组织学观察病变过程。结果随着摄入4NQO浓度的增加,小鼠在同一时期的发病率也随之增加,病变加重,同时随着服用时间的延长,病情逐渐加剧。在实验过程中,可以观察到舌和食管的病变经历了单纯性上皮增生—异常增生—原位癌—浸润性癌这样一个经典的鳞状上皮细胞癌的病变过程。当服用100μg/ml4NQO16周,到第28周时可观察到浸润性癌的病理症状。结论采用4NQO饮水法成功建立了C57BL/6小鼠舌癌和食管癌动物模型,为研究口腔鳞状上皮细胞癌和食管鳞状上皮细胞癌提供了有价值的动物模型。

Objective In order to better study the tongue and esophageal squamous cell carcinoma, we use the chemical carcinogen—4-nitroquinoline-1-oxide （ 4NQO） to established C57BL / 6 mice model of tongue cancer and esophageal cancer. Methods 100 μg/ml,50μg/ml and 10μg/ml of 4NQO was delivered to C57BL / 6 mice by drinking water. During this experiment period,the mice were observed by naked eyes and histological assessment. Results In the experiment,the severity of lesions coresponded to the concentration of 4NQO and the duration of observation,the higher concentration or the longer duration,the more serious lesion. The tongue and esophageal lesions had undergone a classical process of squamous cell carcinoma＇s development which include simple epithelial hyperplasia,dysplasia,in situ carcinoma and invasive cancer. The mice were treated with 100μg/ml 4NQO for 16 weeks,at the 28th week,invasive cancer of tongue and esophageal was able to be monitored . Conclusion 4NQO is successfully used in the C57BL / 6 mice to establish an animal model of tongue cancer and esophageal cancer. This model provides a valuable animal model to study oral SCC and esophageal SCC.