壳聚糖-乙二胺四乙酸纳米粒的制备及小鼠体内^(89)Sr^(2+)促排作用研究

Preparation of Chitosan-EDTA Nanoparticles and the Chelating Effect of Radioactive Strontium in vivo

目的合成壳聚糖-乙二胺四乙酸(CTS-EDTA)纳米粒,用于小鼠体内89Sr2+的加速排泄研究。方法通过CTS-EDTA上的游离氨基与多聚磷酸钠(TPP)的静电作用制备CTS-EDTA纳米粒,并利用CTS-EDTA纳米粒两性离子型螯合物的特点,比较不同药物对体内放射性核素的促排效果差异。结果 CTS-EDTA纳米粒通过透射电镜观察显示可得到粒度均一的球形纳米粒子,通过激光粒度分析仪测得平均粒径为10.18nm。通过对小鼠体内放射性锶的促排研究发现,CEC-Nano和CEC有良好的促排效果,单次用药后30min和2h时相点对沉积于股骨中放射性锶的促排效率都明显高于EDTA-Na2(P<0.05)。多次给药时,CEC-Nano和CEC通过尿、粪排泄放射性锶的作用也优于传统药物EDTA-Na2(P<0.05)。结论通过该实验方法可制备纳米型放射性核素促排剂,为进一步研制广谱放射性核素污染促排剂提供实验依据。

Objective To obtain the CTS-EDTA nanoparticles and investigate their effects of chelating 89Sr2 ＋ in vivo. Method The CTS-EDTA nanoparticles were preparaed by cross-linking CTS-EDTA with polyanion sodium tripolyphosphate（ TPP） . And the zwitterionic chelate of the CTS-EDTA nanoparticles was used for promoting the radionuclides excreted in vivo. Results The CTS-EDTA nanoparticles showed that particle size was uniformity of the spherical nano-particles by TEM,and the average particle size of 10. 18nm by Laser Particle Sizer. we found that CEC-Nano and the CEC had a good chelating effect of radioactive strontium in vivo,after 30min and 2h in the medication and the chelating efficiency of radioactive strontium excretion in the femur was significantly higher than the EDTA-Na2. At multiple doses,the chelating efficiency of CEC-Nano and the CEC through the urinary excretion and feces were better than traditional medicines EDTA-Na2. Conclusion By this experimental method,we can be prepare nanodrugs of chelating radionuclide,it provides a basis for studying the broad-spectrum of radionuclide contamination chelating agents.