半乳糖抗小鼠CD_3单克隆抗体的制备及其结合TIL后的趋肝性研究

PREPARATION OF HEPATIC TARGETING CARRIER GAL ANTI MOUSE CD 3 McAb AND STUDY OF ITS TARGETING PROPERTY AFTER COUPLING WITH TIL

报道以半乳糖为原料制得２－亚氨基－２－甲氧乙基－１－硫代－β－Ｄ－半乳糖苷（ＩＭＥ）后，与抗小鼠ＣＤ３单克隆抗体共价偶联制得半乳糖抗小鼠ＣＤ３单克隆抗体，再与标记３Ｈ－ＴｄＲ的肿瘤浸润性淋巴细胞（ＴＩＬ）结合。经动物试验表明，复合物较单纯ＴＩＬ具有明显的趋肝性，且能较长时间地浓集于小鼠肝脏内，说明半乳糖抗小鼠ＣＤ３单克隆抗体可作为肝靶向载体将抗肿瘤药选择性地导向肝脏，可能为肝癌的生物导向治疗开辟了新的途径。

A hepatic targeting carrier, galactosyl anti mouse CD 3 monoclonal antibody(Gal anti CD 3 McAb),was synthesized by the covalent coupling of a carbohydrate bifunctional reagent, 2 imino 2 methoxyethyl thiogalactose,to anti mouse CD 3 McAb.The carrier was combined with 3H TdR labeled tumor infiltrating lymphocytes(TIL) and the animal tests showed that the TIL combining gal anti CD 3 McAb had much better hepatic targeting trendency than TIL alone. These results indicated that Gal anti CD 3 McAb was probably used as a hepatic targeting carrier of anti tumor drugs. This study created a new way for hepatic cancer biological targeting treatment.