分光光度法测定左旋氧氟沙星的尿药浓度

SPECTROPHOTOMETRIC DETERMINATION OF LEVOFLOXACIN IN HUMAN URINE

用分光光度法测定尿液中左旋氧氟沙星（ＬＶＦＸ）的排泄量。尿样在二甲基亚砜存在下与三氯化铁甲醇溶液反应显色，经甲醇稀释，在４１７ｎｍ波长处直接测定吸光度。方法平均回收率为（１００．５±２．４７）％，ｎ＝９，线性范围（１６．２２～２２７．１４）μｇ／ｍｌ（ｒ＝０．９９９７），ＲＳＤ＜６．０８％。受试者尿中的ＬＶＦＸ１２ｈ总排泄量为给药量的５７．０５％。该法精密度、重现性良好，操作简便，可应用于人尿中ＬＶＦＸ的测定。

To determine levofloxacin (LVFX) concentrations in human urine, the intensities of the orange red colour produced by the drugs with ferric chloride in dimethylsulphoxide methanol medium are measured, λ max is 417 nm, recoveries are (100.5± 2.47)% ( n=9 )and r=0.9997 in concentrations from 16.22￣227.1 μg/ml, RSD < 6.1%, total excreted amount of LVFX in 12 h urine is 57.05%. The method is highly accurate and has been successfully applied to analysis of LVFX in human urine.