HPLC法测定小鼠肝匀浆中乳梨醇的含量

DETERMINATION OF LACTITOL IN MICE LIVER HOMOGENIZATIONS BY HPLC

用高效液相色谱法建立了小鼠肝匀浆中乳梨醇的含量测定方法。乳梨醇的回收率为９９．９％，日内和日间ＲＳＤ均小于１．０％（ｎ＝５）。

A HPLC method was developed for the determination of lactitol in mice liver homogenizations. The analysis was performed by using a Waters Sugar Pak1 column with redistilled water as mobile phase, the column temperature was maintained at 90℃, the differential refractometer was used. The average recovery was 99.9%. The limit of detection was 20 μg/ml (S/N=3). The linear range was 20￣500 μg/ml( r =0.9996). Within day and between day RSD were less than 1.0% ( n=5) . The result showed that lactitol liver was not found in mice.