摘要
目的定量描述肝癌细胞和肝细胞在IV型胶原裱衬表面的粘附特性,探讨细胞粘附力与IV型胶原裱衬浓度及IV型胶原与层粘连蛋白(LN)复合裱衬时LN复合浓度的相关关系。方法采用分步裱衬的方法制作有关裱衬表面,即依次在玻璃表面裱衬多聚赖氨酸和IV型胶原/LN,最后以牛血清白蛋白作用阻断非特异性粘附。细胞粘附力采用微管吸吮技术进行测定。结果细胞粘附力表现出对IV型胶原裱衬浓度较好的依赖性。当裱衬质量浓度从1μg/ml增加至5μg/ml时,肝癌细胞粘附力增加幅度[202×10-10牛顿(N),26%]远远小于肝细胞粘附力增加幅度(1566×10-10N,742%),并呈现饱和效应,Ⅳ型胶原裱衬质量浓度超过2μg/ml时,肝癌细胞粘附力不再增加。LN与IV型胶原复合裱衬并不一定导致肝细胞和肝癌细胞在Ⅳ型胶原裱衬表面的粘附力增加。在特定的复合裱衬质量浓度(0.625μg/ml或1.25μg/ml),LN使肝细胞和肝癌细胞在Ⅳ型胶原裱衬表面的粘附力达到最大值,其余浓度的LN与Ⅳ型胶原复合裱衬均引起细胞粘附力的下降。结论上述方法是定量研究细胞与胶原蛋白粘附特性的有效手段。肝癌细胞与Ⅳ型胶原的粘附特性较之肝细胞发生了明显的变化。L?
Objectives To quantitatively describe the adhesion of
hepatocellular carcinoma (HCC) cells and hepatocytes to collagen /laminin coated surfaces and
to investigate the relevance of cell adhesion forces to the coating concentration of collagen
and, when laminin was cocoated along with collagen , the incorporation concentration of
laminin. Methods A stepwise coating protocol was adopted to prepare the collagen /laminin
coated surfaces, in which polyDlysine and collagen /laminin were successively coated onto
glass surfaces and finally, bovine serum albumin was used to block the nonspecific adhesion.
Cell adhesion forces were measured by means of micropipette aspiration technique. Results
Cell adhesion forces showed good dependence on the coating concentration of collagen .
However, the amplitude for the increase of adhesion forces of HCC cells (20210-10N,26%) was
far more less than that for hepatocytes(1 56610-10N,742%) when coating concentration
increased from 1 g/ml to 5 g/ml. No further increase in the adhesion forces of HCC cells was
observed when the coating concentration of collagen IV increased beyond 2g/ml. The
incorporation of laminin along with 2 g/ml collagen did not readily result in an increase in the
adhesion forces of the two kinds of cells to the surfaces. Among the concentration of laminin
tested, only in certain concentration(0.625 g/ml or1.25 g/ml) did the incorporation of laminin
along with 2 g/ml collagen IV lead the adhesion forces of hepatocytes and HCC cells to the
coated surfaces to reach their corresponding maximum values, otherwise this incorporation led
the adhesion forces to decrease. Conclusion The present method is efficient in investigating
cellmatrix adhesion. The cell adhesion behavior to collagen for HCC differs obviously from
those for normal hepatocytes. Laminin might be an important factor for the regulation of cell
adhesion forces to matrix components
出处
《中华医学杂志》
CAS
CSCD
北大核心
1999年第5期369-372,共4页
National Medical Journal of China
基金
国家自然科学基金
关键词
肝细胞癌
Ⅳ型胶原
肝瘤
层粘连蛋白
粘附
Carcinoma, hepatocellular Collagen
LamininAdhesionMicropipette aspiration