珊瑚复合人工骨骨诱导活性的实验研究

EXPERIMENTAL STUDY ON OSTEOINDUCTION OF CORAL COMPOSITED ARTIFICIAL BONE

目的弥补单纯珊瑚无骨诱导活性、骨修复能力弱等缺陷，为临床提供理想的骨移植替代材料。方法将珊瑚与胶原和重组人骨形成蛋白２（ｒｈＢＭＰ２）复合，制备珊瑚／胶原／ｒｈＢＭＰ２复合人工骨，将其植入大鼠背部肌肉陷窝，以珊瑚／胶原和珊瑚／ｒｈＢＭＰ２复合人工骨植入作对照；取材后通过组织学方法和图像分析评价其骨诱导活性。结果珊瑚／胶原／ｒｈＢＭＰ２植入后１周，在局部诱导出软骨细胞分化和软骨基质形成；植入后４周，形成含骨髓的板层骨；诱导成骨的量有明显的ｒｈＢＭＰ２剂量依赖性（Ｐ＜０．０１）；而珊瑚／胶原和珊瑚／ｒｈＢＭＰ２植入区均无骨或软骨形成。结论珊瑚／胶原／ｒｈＢＭＰ２复合人工骨具有良好的异位诱导成骨活性，是一种较理想的骨移植替代材料。

Objective To improve the osteoinduction of coral and provide a perfect bone graft substitute for clinical bone defects.Methods By combining coral with collagen and recombinant human bone morphogenetic protein 2(rhBMP 2),coral/collagen/rhBMP 2 composite was obtained.The composite was implanted into the back muscle pouches of mice ,and coral/collagen or coral/rhBMP 2 were implanted as control.The osteoinduction of the composite was assessed by histology and image analysis system.Results The chondrocyte differentiation and matrix formation were observed in local sites after one week,lamellar bone with bone marrow were formed after 4 weeks,and coral were absorbed partially.The quantity of osteoinduction was time related and rhBMP 2 dose related(P<0.01).Coral/collagen and coral/rhBMP 2 implants did not show any bone or cartilage formation.Conclusion The coral/collagen/rhBMP 2 composite possesses a superior osteoinduction and will be a new type of bone substitute to be used in orthopedic and maxillofacial surgery.