H-point计算分光光度法研究及其在复方盐酸丁卡因注射液中的应用

Determinaton of Compound Injection of Tetracaine Hydroch loride by Using Different H point Standard Additions Methods

介绍几种Ｈ－ｐｏｉｎｔ法，并与常见的计算分光光度法进行了比较。将Ｈ－ｐｏｉｎｔ法用于复方盐酸丁卡因注射液测定的数据处理，表明Ｈ－ｐｏｉｎｔ法有效地消除干扰，有较高的精密度和准确度。盐酸丁卡因回收率为：Ｈ－ｐｏｉｎｔ法１０１．０％，ＲＳＤ为２．７％（ｎ＝７），Ｋ系数Ｈ－ｐｏｉｎｔ法９９．１％，ＲＳＤ为１．８％（ｎ＝７），三波长Ｈ－ｐｏｉｎｔ法９９．６％，ＲＳＤ为１．４％（ｎ＝７）。

A set of new analytical methods for the determinaton of tetracaine hydrochloride in compound Injection of Tetracaine Hydrochloride are proposed by using different H point standard additions method.By proper selection of the combination of wavelengths,the satisfactory result was obtained with the recovery as 101.0 ( RSD =2.7%; n =7),99.1% (K ratio H point standard additions method RSD=1.8%; n =7),100.9%(Three wavelenth H point standard additions method, RSD=1.4%; n =7),while the interferences of the background and p benzaminic acid are obviated.Compared with the relative methods,the precision and the accuracy of the assay have been improved.