摘要
报道了用分段改变洗脱液浓度的方法,将反式番红花甙和顺式番红花甙类化合物进行HPLC分离。本法以Nova-PakC18(3.9mm×150mm,4μ)柱为固定相,甲醇-水(1%HAc)1ml/min进行梯度洗脱,在440nm,308nm,250nm波长下同时检测,番红花酸(crocetin)、四种反式番红花甙(trans-crocins)和顺式番红花甙(cis-crocins)得到了较好的分离。用视网膜电镜法观察了大鼠视网膜中心动脉缺血后,番红花酸和番红花甙1对血流量恢复的影响。
Crocins are the major components in saffron. They are the esters of crocetin (8,8′ diapo Ψ,Ψ′ carotenedioic acid) and it′s very difficult to separate them. HPLC was used to separate trans and cis crocin in this paper. The conditions of HPLC were: Nova Pak C 18 (3.9 mm×150 mm, 4 μ) column; Methanol H 2O(1% HAc) as mobile phase in which the concentration of methanol was changed at different rates in three parts; Detection wavelength is 440 nm, 308 nm, 250 nm.Trans and cis crocins were separated at shorter time. Crocin 1 and crocetin were found to significantly increase the blood flow in the retina and choroid and to facilitate retinal function recovery by electroretinography.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1999年第2期108-111,共4页
Journal of China Pharmaceutical University
基金
国家自然科学基金
关键词
番红花甙
分离
药理活性
高效液相色谱
Crocins
HPLC
Electroretinography
Separate
Pharmacological activity