α-干扰素对膀胱癌细胞多药耐药性逆转的研究

Reversal of multidrug resistance by α IFN in bladder cancer cell lines

目的研究α干扰素（αＩＦＮ）对膀胱癌多药耐药性的逆转作用。方法应用ＭＴＴ法检测阿霉素（ＡＤＭ）的细胞杀伤作用。荧光法检测细胞内阿霉素浓度和斑点杂交法检测ｍｄｒ１ｍＲＮＡ相对含量。结果５００ＩＵ／ｍｌαＩＦＮ能明显增强阿霉素对耐药细胞ＢＩＵ８７／ＡＤＭ的杀伤作用，但不能增强阿霉素对敏感细胞ＢＩＵ８７的杀伤作用；αＩＦＮ能增加阿霉素在ＢＩＵ８７／ＡＤＭ细胞内的积聚，但不改变ｍｄｒ１ｍＲＮＡ在细胞内表达量。结论αＩＦＮ对膀胱癌细胞多药耐药性具有逆转作用，其机制与αＩＦＮ增加细胞内抗癌药物浓度有关，与增加ｍｄｒ１ｍＲＮＡ表达量无关。

Objective To study the reversal of multidrug resistance by α IFN in bladder cancer cell line. Methods The effect of recombinant human α interferon (αIFN) on the cytotoxicity of adriamycin(ADM) in human bladder cancer cell line BIU 87 and its multidrug resistant counterpart BIU 87/ADM were studied by MTT assay. ADM accumulation and level of mdr1 mRNA in above cell lines were also detected by spectrofluometric and dot blot analysis respectively. Results α IFN (500IU/ml) increases the cytotoxicity effect and the accumulation of ADM in BIU 87/ADM cell line, but had no effect on the cytotoxicity of ADM to BIU 87 cell line, the level of mdr1 mRNA being unchanged. Conclusions α IFN has significant effects on the reversal of multidrug resistance of human bladder cancer cell line.