血管内皮细胞与大肠癌细胞粘附后肌动蛋白Actin表达的研究

Study on the expression of Actin after vascular endothelial cell adhered to colorectal cancer cell

目的 探讨血管内皮细胞与大肠癌细胞粘附后,内皮细胞内肌动蛋白Actin表达的情况,分析其表达与肿瘤转移的关系.方法 采用细胞培养、免疫组化及免疫荧光染色技术检测单独培养的内皮细胞及与不同转移能力的人大肠癌细胞系LoVo细胞、HT29细胞共培养之内皮细胞内Actin的表达.结果 单独内皮细胞培养组的细胞内Actin主要分布在细胞的周边,分布均匀、排列整齐,细胞间连接紧密,没有间隙形成,荧光强度较强;而内皮细胞与HT29细胞共培养组中内皮细胞周边的Actin基本消失,出现应力纤维,排列呈明显的极向分布,细胞间隙形成,荧光强度减弱;内皮细胞与LoVo细胞共培养组变化则更显著（26.55±15.23和15.78±11.54, P＜0.05）.结论 大肠癌细胞的粘附可使内皮细胞的骨架蛋白发生改变和分布异常,高转移能力的癌细胞对内皮细胞Actin的影响更为显著.

Objective To detect the expression of Actin in vascular endothelial cell （EC） after colorectal carcinoma cell adhered to EC to analyze the expression of Actin and metastasis of tumor. Methods Cell culture, immunohistochemistry, used to detect the expression of Actin in ECs after we cultivated ECs solely and ECs co - cultured with HT29 cells and LoVo cells respectively. Results In solely cultured ECs, Actin is at margin of cell primarily, well - proportioned distributing, arranging tidily, intercellular connect is close, there is no gap formed. Fluorescence intensity is strong. However, .in groups that we cultured ECs together with colorectal cancer cells, peripheral Aetin of EC disappear, and appearing stress fibre （latching, shortening, turbulence, polar distributing）, going with intercellular gap. Fluorescence intensity weakens, especially in group of ECs cocultured with Lovo cells（26.55 ~ 15.23 vs 15.78± 11. 54, P 〈 0.05）. Conclusion ECs and colorectal cancer cells adhesion, Actin in ECs will change, thus affect distributing of Actin in ECs, particularly ECs adhered to high metastatic cancer cells.