铜绿假单胞菌耐药性及氟喹诺酮类耐药相关基因的研究

Study on drug resistance and resistance gene of Fluoroquinolones in Pseudomonas aeruginosa

目的 了解医院临床分离的铜绿假单胞菌耐药情况及氟喹诺酮类相关耐药基因存在状况.方法 用BD Phoenix100全自动微生物分析仪进行细菌鉴定和药物敏感试验,用聚合酶链反应（PCR）检测parC、gyrA两种氟喹诺酮类耐药相关基因.结果 70株铜绿假单胞菌呈现多重耐药,对环丙沙星、左旋氧氟沙星的耐药率分别为51.4%、50.0%,其余11种的耐药率在10.0%～100.0%.在70株铜绿假单胞菌中,39（55.7%）株分离菌检出parC基因,18（25.7%）株分离菌检出gyrA基因.结论 临床分离的铜绿假单胞菌多重耐药严重,携带parC和gyrA基因是本组试验菌株对氟喹诺酮类抗生素耐药的重要机制.

Objective To investigate the drug resistanee and resistance gene associated with fluoroquinoione ha Pseudomonas aeruginosa. Methods BD phoenix 100 automatic microbial analysis was used for species identification and drug susceptibility test. PGR assay was used t. amplify fluoroquinolone coding genes including par C and gyr A. Resulls 70 strains were exhibited multiple - drug - resistance. The resistant rate to eiprofloxaein and levofloxacin were 51.5% and 50.0%, respectively. The resistant rate to other antimicrebial agents were 10.3% to 100.0% . In the 70 strains, the positive strains of par C were 39（55.7 % ）, and that of gyr A were 18（25.7 % ）. Conclusions The multiple - drug - resistance of Pseudomonas aemginosa isolated from clinical samples is severely. The emergence of par C and gyr Amay be the essential resistant mechanism of Fluoroquinoibnes in the tested Pseudomonas aeruginosa.