滤纸干血片唐氏综合征产前筛查AFP和β-hCG检测的影响因素

Influential factor of AFP and β-hCG dection by dried blood spot in Down＇s syndrome prenatal screening

目的 探讨滤纸干血片唐氏综合征产前筛查过程中可能影响AFP和β-hCG测定结果的因素.方法 观察不同的洗脱时间、不同保存条件和取样部位、末梢血/静脉血干血片等因素对滤纸干血片检测结果的影响.结果 末梢血和静脉血滤纸干血片检测结果相同.血片边缘取样和中央取样对检测结果没有影响.滤纸干血片完全洗脱时间为1小时.在25℃放置7天时,β-hCG检测结果偏高.结论 AFP和β-hCG在滤纸干血片中可稳定存在.采用冷链运输,按照标准流程进行操作,可保证滤纸干血片唐氏综合征产前筛查质量.

Objective To investigate the influence ,factors of dried blood spot determination of AFP and β- hCG during prenatal screening of Down＇ s syndrome. Methods The influential factors of assay such as collecting blood from finger or venus, different elution time, sampling site, specimen store condition were observed. Results peripheral blood spot or venous blood spot and different sampling site have no influence to the detection. The entire elution time is one hour. The concentration of β - hCG is higher when the blood spot stay under 25 ℃ for 7 days. Conclusion AFP andβ- hCG can stay in the dried spot stably. To assure blood spot sample are transported under cold condition and the test are operated according to SOP , the screening quality can be assured effectively.