玻璃苣总黄酮提取工艺的研究

Extraction Technique of Total Flavonoids from Borage Officinales L.

采用正交实验研究各因素的影响大小确定玻璃苣中总黄酮的最佳提取条件,通过紫外分光光度法测定总黄酮的含量。乙醇浓度对玻璃苣中总黄酮的提取影响作用最大,玻璃苣中总黄酮的最佳提取工艺为:乙醇浓度75%、温度90℃、提取次数为4次、料液比为1:6、提取时间为1.5h·次^(-1)。本研究可为玻璃苣总黄酮工业化提供理论依据。

The extraction process of total flavonoids in Borage officinales L. was optimized by single factor experiments. The content of total flavc oids was determined by UV spectrophotometry. The influence of each factor was measured in orthogonal experiments. The content of ethanol had the greatest effect on the extraction of total flavonoids. The optimal conditions were as follows： 75% ethanol, 90℃, 1 ： 6 （W/V） and extracting 4 times （1.5h · eachtime-1）. This study provides the rational foundation for the industrial production.