摘要
建立了一种基于CdSe/CdS量子点(QDs)标记技术测定微囊藻毒素-LR(MC-LR)的方法。MC-LR抗体经1-乙基-3(3-二甲基胺基丙基)碳二亚胺盐酸盐(EDC)活化,与经巯基乙酸修饰的CdSe/CdS量子点进行共价连接,获得的QDs生物共轭体在待测物MC-LR的作用下发生荧光猝灭现象,荧光猝灭程度与MC-LR的浓度呈线性关系。该方法操作简单、检测速度快、检出限低达6.9×10^(-11)mol/L。
A new method for the determination of microcystinqeucine-arginine （MC-LR） in water samples was developed based on the fluorescence quenching of bioconjugates between CdSe/CdS quantum dots （QDs） and MC-LR antibody in the presence of the analyte. After activated by 1-ethyl-3- （3-dimethyllaminopropyl） carbodiimidc hydrochloride,monoclonal anti-MC-LR antibody was covalently conjugated to the modified QDs by 2-mercaptoacetic acid to form their bioconjugates. The fluorescence intensity of the bioconjugates between QDs and monocional anti-MC-I.R antibody was quenched in the presence of MC-LR. A linear relationship between the percentage of fluorescence quenching and MC-LR concentrations was observed. This method is easy, fast with limit of detection of 6.9×10 -11mol · L-1（3σ）.
出处
《光谱实验室》
CAS
CSCD
北大核心
2010年第6期2354-2358,共5页
Chinese Journal of Spectroscopy Laboratory
基金
国家自然科学基金项目(20345006
20575043)
关键词
量子点
微囊藻毒素
荧光光谱
Quantum Dots
Mierocystin-LR
Fluorescence Spectroscopy
