白蛋白结合型紫杉醇对人骨肉瘤OS-732细胞的体外抑制作用观察

In vitro inhibiting effect of albumin-bound paclitaxel on human osteosarcoma cell OS-732

目的观察白蛋白结合型紫杉醇(Abraxane)对人骨肉瘤OS-732细胞的体外抑制作用。方法取传代第3天、处于指数增殖期的人骨肉瘤OS-732细胞,分别加入不同浓度的阿霉素、紫杉醇(泰素)和Abraxane培养,于培养24、72、144 h时用MTT法测定细胞的光密度值(OD值)并计算细胞毒性指数(CI),倒置相差显微镜观察细胞形态,流式细胞术检测细胞周期。结果骨肉瘤OS-732细胞加入Abraxane后,OD值下降,以加入100、1 000μg/ml的Abraxane组及培养72、144 h时下降明显,CI则相应升高,同时细胞变小、核碎裂,G2～M期细胞的比例逐渐增加;除100μg/ml浓度处理24 h和72 h后,泰素处理组的CI高于Abraxane组外,两组相同浓度、相同处理时间的CI相近;最高剂量Abraxane和最高剂量阿霉素组的CI也相近。结论 Abraxane在体外对骨肉瘤OS-732细胞有显著的抑制作用,能将骨肉瘤细胞阻滞于G2～M期。Abraxane与泰素和阿霉素的细胞毒性相似。

Objective To evaluate the inhibiting effect of albumin-bound paclitaxel（Abraxane） on human osteosarcoma cell OS-732 in vitro.Methods human osteosarcoma OS-732 cells were cultured for 3 days in proliferation period.Adriamycin,paclitaxel（Taxol） and Abraxane were used to treat osteosarcoma cells for 24 h,72 h and 144 h.MTT was used to evaluate the cytotoxic effects through measuring optical density（OD） and cytotoxity index（CI）.Cellular morphologic changes were observed with phase contrast microscope.FCM was performed to detect cell cycle phase and DNA ploidy in osteosarcoma cells.Results The OD of cells treated by 100 μg/ml and 1 000 μg/ml Abraxane descended significantly over time,and the CI value elevated as well,while cells shrinked and nuclear chipped.With the increasing of drug concentration,G2～M phase cells increased gradually.The osteosarcoma cells received Abraxane showed similar CI with that in Taxol group,except that cells treated by 100 μg/ml concentration for 24 h and 72 h.The CI in maxium concentration Abraxane group was similar with that in maxium concentration Adriamycin group.Conclusions Abraxane has anti-tumor activity in human osteosarcoma cell OS-732 in vitro.The cells showe G2 ～ M cell cycle arrest after treatment.The cytotoxity of Abraxane is similar with Adriamycin and Taxol.