血清IgE和最大呼气中期流速在儿童哮喘中的临床意义

Clinical implications of serum total IgE and maximal midexpiratory flow rate in asthmatic children

目的探讨支气管哮喘儿童血清总IgE与最大呼气中期流速变化的意义。方法选择支气管哮喘患儿23例和正常对照组16例为研究对象,采用双抗体夹心ELISA法检测血清总IgE水平,外周血嗜酸细胞计数采用全自动血液分析仪计数法。同时检测肺功能指标中的最大呼气中期流速。结果支气管哮喘患儿血清总IgE水平为(242.50±54.48)IU/ml,明显高于正常对照组(50.40±8.58)IU/ml,差异有统计学意义(P<0.01)。与正常对照组相比,哮喘组的外周血嗜酸细胞计数明显升高〔(300.00±84.05)vs(140.55±23.35)个/μl,P<0.05〕,而最大呼气中期流速显著降低〔(75.40±25.48)%vs(92.32±28.20)%,P<0.01〕。线性回归分析表明血清总IgE水平与外周血嗜酸细胞计数、体质指数、最大呼气中期流速无相关性。结论支气管哮喘患儿血清总IgE水平和外周血嗜酸细胞计数明显升高。IgE在儿童哮喘的发病机制中具有重要作用。

Objective To evaluate the changes of serum total IgE and maximal midexpiratory flow rate in children with asthma. Methods Eligible 23 children with bronchial asthma served as asthmatic group,and 16 healthy subjects served as control group. The body mass index （BMI） ,pulmonary function tests including maximal midexpiratory flow rate （ MMEF）, peripheral blood eosinophil count, and serum total IgE level were measured in all subjects. Serum total IgE level was assayed with double-antibody sandwich ELISA, and eosinophil count was detected by automatic blood analyzer counting. The results mentioned above in two groups were compared and analyzed. Results Compared with control group, the levels of serum total IgE significantly elevated [ （ 242. 50 ±54. 48） vs （50. 40 ± 8.58 ） IU/ml, P 〈 0. 01 ], the peripheral blood eosinophil count significantly increased [ （ 300. 00 ± 84. 05 ] vs （ 140. 55 ± 23.35 ）/μl, P 〈 0. 05 ], MMEF obviously decreased [（75.40 ±25.48） % vs （92. 32 ±28.20） % ,P 〈 0.01 ] in asthmatic group. Linear regression analysis showed that the serum IgE level was not correlated with eosinophil count,BMI and the changes of MMEF. Conclusions Serum total IgE and peripheral blood eosinophil count significantly increased in children with asthma, and the serum total IgE plays an important role in the asthmatic pathogenesis.