摘要
目的:探讨SHH和Wnt信号及其下游信号分子Gli-1和Axin2在人子宫内膜癌中的表达及相关性,分析其在子宫内膜癌发生发展中的作用。方法:应用免疫组织化学方法检测正常人子宫内膜、内膜增生和子宫内膜癌37例中SHH和Wnt信号的表达,采用RT-PCR方法检测上述组织中Gli-1和Axin2的表达情况。结果:内膜增生组和癌组SHH和Wnt的表达均强于正常组(P<0.05)。Gli-1的表达在正常组和子宫内膜增生组类似,在癌中表达较低;Axin2的表达在3组中类似都较低,差异无统计学意义(P>0.05)。结论:SHH和Wnt的表达均与子宫内膜癌的发生密切相关,Gli-1主要参与子宫内膜癌进展变化。
Objective：The present study was undertaken to examine the expression and functional involvement of the SHH and Wnt pathway in endometrial tissues.Method：The expression of SHH and Wnt was examined in normal endometrial tissues,hyperplasia and carcinomatous endometrium with Immunohistochemistry.Axin2 and Gli-1 as effective factors of Wnt and SHH signaling on the en-dometrial carcinoma were also examined by RT-PCR.Results：The expression of SHH and Wnt was weak in normal endometrium,but increased in endometrial hyperplasia and carcinoma stepwisely with significant differences.The expression of Gli-1 mRNAs was similar in normal endometrial tissues and the proliferative phase,with a weak expression in endometrial carcinomas.The expression of Axin2 mRNAs was similar by weak in the three groups,with no significant differences.Conclusions：The abnormal activation of SHH and Wnt pathway is involved in the endometrial carcinoma possibly,and Gli-1 mainly takes part in the evolvement of the endometrial carcinoma.
出处
《交通医学》
2010年第5期479-482,共4页
Medical Journal of Communications
