急性坏死性胰腺炎肺损伤大鼠高迁移率族蛋白B1的表达及意义

The Expression and Significance of HMGB1 in Rats with Acute Necrotizing Pancreatitis-associated Lung Injury

目的:观察急性坏死性胰腺炎(ANP)肺损伤大鼠血清高迁移率族蛋白B1(HMGB1)的表达,探讨HMGB1在急性坏死性胰腺炎并发急性肺损伤(ALI)中的意义及机制。方法:将100只SD大鼠随机分成假手术组(SO组)和模型组(ANP组)。各组分别在6h、12h、24h、36h、48h采用酶联免疫吸附法测定血清中HMGB1,同时行动脉血气分析,观察胰腺和肺组织病理形态学的改变。结果:ANP组血清HMGB1从12h开始明显上升,48h达到高峰,与SO组比较,差异有统计学意义(P<0.01)。随着胰腺损伤的逐渐加重,肺损伤程度也在逐渐加重,PaO2进行性下降,PaCO2进行性升高。结论:血清HMGB1的增高与急性坏死性胰腺炎致ALI病情严重程度呈正相关。

Objective：To investigate the role and the passible mechanism of HMGB1 in acute necrotizing pancreatitis-associated lung injury in rats.Methods：One hundred Sprague-Dawley rats were randomly divided into two groups： sham operation group（SO group）and acute necrotizing pancreatitis group（ANP group）.Animals in each group were sacrificed at 6 h,12 h,24 h,36 h and 48 h af-ter operations respectively.The concentrations of serum HMGB1 were determined by enzyme-linked immunosorbent assay（ELISA）.Blood gas analysis was performed.Moreover,pancreatic and pulmonary pathomorphology was compared between the two groups.Re-sults：In the ANP group,serum HMGB1 increased markedly at 6 h,and culminated at 48 h.There was a significant difference in serum HMGB1 concentrations between the ANP group and the SO group（P〈0.01）.The severity of lung injury was parallel with the severity of pancreas injury.Conclusions：The extent of serum HMGB1 increase is positively correlated with the severity of ALI.